Composite

Part:BBa_K4941107:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-12)


pylxp-ylER-StYida


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4319
    Illegal PstI site found at 6895
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 6895
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1788
    Illegal XhoI site found at 2186
    Illegal XhoI site found at 5312
    Illegal XhoI site found at 5345
    Illegal XhoI site found at 10438
    Illegal XhoI site found at 10467
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4319
    Illegal PstI site found at 6895
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4319
    Illegal PstI site found at 6895
    Illegal NgoMIV site found at 5500
    Illegal NgoMIV site found at 11324
    Illegal AgeI site found at 2665
    Illegal AgeI site found at 4442
    Illegal AgeI site found at 6270
    Illegal AgeI site found at 9853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1634
    Illegal BsaI site found at 9032
    Illegal BsaI site found at 10609
    Illegal BsaI.rc site found at 1049
    Illegal BsaI.rc site found at 2988
    Illegal BsaI.rc site found at 4765
    Illegal BsaI.rc site found at 10524
    Illegal BsaI.rc site found at 10807
    Illegal SapI site found at 1936
    Illegal SapI.rc site found at 757
    Illegal SapI.rc site found at 10055


Design Notes

none


Source

yarrowia lipolytica

References